Isabel of Portugal

Isabel of Portugal (24 October 1503 – 1 May 1539) was the empress consort and queen consort of her cousin Charles V, Holy Roman Emperor, who was the ruler of the Spanish Empire. She was Queen of Spain and Germany, and Lady of the Netherlands from 10 March 1526 until her death in 1539, and became Holy Roman Empress and Queen of Italy in February 1530. She was the regent of Spain because of her husband's constant travels through Europe, ensuring that the kingdom remained independent of imperial policies.

Issue

 * 1) Philip II of Spain (21 May 1527 – 13 September 1598)
 * 2) Archduchess Maria of Austria, Infanta of Spain (21 June 1528 – 26 February 1603) married Maximilian II, Holy Roman Emperor and had issue.
 * 3) Archduke Ferdinand of Austria, Infante of Spain (	22 November 1529 – 13 July 1530) died in infancy.
 * 4) Stillborn son (29 June 1534)
 * 5) Archduchess Joanna of Austria, Infanta of Spain (26 June 1535 – 7 September 1573) married João Manuel, Hereditary Prince of Portugal and had idssue.
 * 6) Miscarried son (21 April 1539)